Difference between revisions of "Part:BBa K2686001:Experience"
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| − | The part was designed and constructed in the original pET14 vector from <bbpart>K2686001</bbpart>. The sequence was confirmed using Sanger sequencing. The part was expressed in the pET14 vector using a BL21 DE3 TX-TL (Sun et al., 2013) cell free expression system. First, multiple SDS-PAGE experiments indicate that our expressed protein is at the correct hypothetical size, which was further confirmed by lysine BODIPY tRNA expression (32.16calckDa for the monomer and 1.93MDa for the 60-mer). | + | The part was designed and constructed in the original pET14 vector from <bbpart>K2686001</bbpart> (Cassidy Armstutz et al., 2016, Addgene plasmid #86405). The sequence was confirmed using Sanger sequencing. The part was expressed in the pET14 vector using a BL21 DE3 TX-TL (Sun et al., 2013) cell free expression system. First, multiple SDS-PAGE experiments indicate that our expressed protein is at the correct hypothetical size, which was further confirmed by lysine BODIPY tRNA expression (32.16calckDa for the monomer and 1.93MDa for the 60-mer). |
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===Applications of BBa_K2686001=== | ===Applications of BBa_K2686001=== | ||
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<!-- DON'T DELETE --><partinfo>BBa_K2686001 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K2686001 EndReviews</partinfo> | ||
| + | ===References=== | ||
| + | Cassidy-Amstutz, C., Oltrogge, L., Going, C., Lee, A., Teng, P., Quintanilla, D., East-Seletsky, A., Williams, E. and Savage, D. (2016). Identification of a Minimal Peptide Tag for in Vivo and in Vitro Loading of Encapsulin. Biochemistry, 55(24), pp.3461-3468. | ||
Latest revision as of 22:31, 17 October 2018
The part was designed and constructed in the original pET14 vector from K2686001 (Cassidy Armstutz et al., 2016, Addgene plasmid #86405). The sequence was confirmed using Sanger sequencing. The part was expressed in the pET14 vector using a BL21 DE3 TX-TL (Sun et al., 2013) cell free expression system. First, multiple SDS-PAGE experiments indicate that our expressed protein is at the correct hypothetical size, which was further confirmed by lysine BODIPY tRNA expression (32.16calckDa for the monomer and 1.93MDa for the 60-mer).
Applications of BBa_K2686001
User Reviews
UNIQ1ab0f943b18f902c-partinfo-00000001-QINU UNIQ1ab0f943b18f902c-partinfo-00000002-QINU
References
Cassidy-Amstutz, C., Oltrogge, L., Going, C., Lee, A., Teng, P., Quintanilla, D., East-Seletsky, A., Williams, E. and Savage, D. (2016). Identification of a Minimal Peptide Tag for in Vivo and in Vitro Loading of Encapsulin. Biochemistry, 55(24), pp.3461-3468.