Difference between revisions of "Part:BBa K2533000"
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<h1>'''Characterization'''</h1> | <h1>'''Characterization'''</h1> | ||
This is a two section for lactate produce and transfer part. | This is a two section for lactate produce and transfer part. | ||
| − | [[File:T--HUST-China--2018-tonglu-ldhD-lldP.png |400px|thumb|center|Figure1 | + | [[File:T--HUST-China--2018-tonglu-ldhD-lldP.png |400px|thumb|center|Figure1. ldhD-lldP]] |
<h2>DNA Gel Electrophoretic</h2> | <h2>DNA Gel Electrophoretic</h2> | ||
After we link ldhD and lldP successfully, we design several tags and then add these tags through PCR with an intention to confirm the expression of ldhD and lldP. As the figure shows, we get the genetic stripe about 3000bp which means the PCR is successful. | After we link ldhD and lldP successfully, we design several tags and then add these tags through PCR with an intention to confirm the expression of ldhD and lldP. As the figure shows, we get the genetic stripe about 3000bp which means the PCR is successful. | ||
| − | [[File:T--HUST-China--2018-jiaotu-ldhD-ldhDC.jpeg|400px|thumb|center|Figure2 | + | [[File:T--HUST-China--2018-jiaotu-ldhD-ldhDC.jpeg|400px|thumb|center|Figure2. Expression of ldhD-lldP]] |
<h2>Metabolic figure</h2> | <h2>Metabolic figure</h2> | ||
| − | [[File:T--HUST-China--2018-metabolic figure1.png|400px|thumb|center|Figure3 | + | [[File:T--HUST-China--2018-metabolic figure1.png|400px|thumb|center|Figure3. Metabolic figure of cyanobacteria with ldhD-lldP]] |
As the figure shows, with the help of light and PSI, Synechocystis sp. PCC 6803(cyanobacteria) with ldhD-lldP absorb CO2 into Calvin cycle and produce pyruvate. And then ldhD can transfer pyruvate into lactate which will be transported to the environment by lldP. | As the figure shows, with the help of light and PSI, Synechocystis sp. PCC 6803(cyanobacteria) with ldhD-lldP absorb CO2 into Calvin cycle and produce pyruvate. And then ldhD can transfer pyruvate into lactate which will be transported to the environment by lldP. | ||
Revision as of 22:18, 17 October 2018
RBS-ldhD-RBS-lldP
ldhD can produce lactate, and lldP can release it from cytoplasm to the environment.
Characterization
This is a two section for lactate produce and transfer part.
DNA Gel Electrophoretic
After we link ldhD and lldP successfully, we design several tags and then add these tags through PCR with an intention to confirm the expression of ldhD and lldP. As the figure shows, we get the genetic stripe about 3000bp which means the PCR is successful.
Metabolic figure
As the figure shows, with the help of light and PSI, Synechocystis sp. PCC 6803(cyanobacteria) with ldhD-lldP absorb CO2 into Calvin cycle and produce pyruvate. And then ldhD can transfer pyruvate into lactate which will be transported to the environment by lldP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 780
Illegal BglII site found at 1325 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1461
Illegal AgeI site found at 1924
Illegal AgeI site found at 2362
Illegal AgeI site found at 2521
Illegal AgeI site found at 2533 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1035