Difference between revisions of "Part:BBa K2719004:Experience"
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===Applications of BBa_K2719004=== | ===Applications of BBa_K2719004=== | ||
| − | <p>This part was optimized <i>Escherichia coli</i> BL21 (DE3) so it could be transformed into it and also cloned in pSB1C3. For proving it presence it was necessary to make two agarose gels, one for the entire plasmid and the other one for the restriction part in order to observe the insert. (Figure 2)</p> | + | <p>This part was optimized <i>Escherichia coli</i> BL21 (DE3) so it could be transformed into it and also cloned in pSB1C3. For proving it presence it was necessary to make two agarose gels, one for the entire plasmid (figure 2) and the other one for the restriction part in order to observe the insert. (Figure 3)</p> |
| + | [[file:T--TecCEM--TCSD5Colonies.png|500px]] | ||
| + | <p><i>Figure 1.</i> Colonies transformed with Tenascin Fibronectin domains.</p> | ||
| + | [[file:T--TecCEM--GelK2719004.png|500px]] | ||
| + | <p><i>Figure 2.</i> 0.85% agarose gel with GelRed, MW)NEB 1Kb Plus DNA Ladder, 1)Extraction Tenascin 5 Fibronectin domains (BBa_K2719004), 2)Extraction Tenascin 5 Fibronectin domains (BBa_K2719004) duplicate.</p> | ||
| + | [[file:T--TecCEM--ResK2719004.png|500px]] | ||
| + | <p><i>Figure 3.</i> 0.85% agarose gel with GelRed, MW)2-Log DNA Ladder, 8)Restriction Tenascin 5 Fibronectin domains with NotI (BBa_K2719004).</p> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 22:17, 17 October 2018
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Applications of BBa_K2719004
This part was optimized Escherichia coli BL21 (DE3) so it could be transformed into it and also cloned in pSB1C3. For proving it presence it was necessary to make two agarose gels, one for the entire plasmid (figure 2) and the other one for the restriction part in order to observe the insert. (Figure 3)
Figure 1. Colonies transformed with Tenascin Fibronectin domains.
Figure 2. 0.85% agarose gel with GelRed, MW)NEB 1Kb Plus DNA Ladder, 1)Extraction Tenascin 5 Fibronectin domains (BBa_K2719004), 2)Extraction Tenascin 5 Fibronectin domains (BBa_K2719004) duplicate.
Figure 3. 0.85% agarose gel with GelRed, MW)2-Log DNA Ladder, 8)Restriction Tenascin 5 Fibronectin domains with NotI (BBa_K2719004).
User Reviews
UNIQ56f4e329490dd26f-partinfo-00000000-QINU UNIQ56f4e329490dd26f-partinfo-00000001-QINU