Difference between revisions of "Part:BBa K2719004:Experience"
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[[file:T--TecCEM--GelK2719004.png|500px]] | [[file:T--TecCEM--GelK2719004.png|500px]] | ||
<p><i>Figure 2.</i> 0.85% agarose gel with GelRed, MW)NEB 1Kb Plus DNA Ladder, 1)Extraction Tenascin 5 Fibronectin domains (BBa_K2719004), 2)Extraction Tenascin 5 Fibronectin domains (BBa_K2719004) duplicate.</p> | <p><i>Figure 2.</i> 0.85% agarose gel with GelRed, MW)NEB 1Kb Plus DNA Ladder, 1)Extraction Tenascin 5 Fibronectin domains (BBa_K2719004), 2)Extraction Tenascin 5 Fibronectin domains (BBa_K2719004) duplicate.</p> | ||
| + | [[file:T--TecCEM--ResK2719004.png|500px]] | ||
| + | <p><i>Figure 3.</i> 0.85% agarose gel with GelRed, MW)NEB 1Kb Plus DNA Ladder, 4)Restriction Tenascin 5 Fibronectin domains with NotI (BBa_K2719004).</p> | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 22:12, 17 October 2018
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K2719004
This part was optimized Escherichia coli BL21 (DE3) so it could be transformed into it and also cloned in pSB1C3. For proving it presence it was necessary to make two agarose gels, one for the entire plasmid (figure 2) and the other one for the restriction part in order to observe the insert. (Figure 3)
Figure 1. Colonies transformed with Tenascin Fibronectin domains.
Figure 2. 0.85% agarose gel with GelRed, MW)NEB 1Kb Plus DNA Ladder, 1)Extraction Tenascin 5 Fibronectin domains (BBa_K2719004), 2)Extraction Tenascin 5 Fibronectin domains (BBa_K2719004) duplicate.
Figure 3. 0.85% agarose gel with GelRed, MW)NEB 1Kb Plus DNA Ladder, 4)Restriction Tenascin 5 Fibronectin domains with NotI (BBa_K2719004).
User Reviews
UNIQcc83f3cfe9e2ba98-partinfo-00000000-QINU UNIQcc83f3cfe9e2ba98-partinfo-00000001-QINU