Difference between revisions of "Part:BBa K2725016"
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Serine amber suppressor tRNA gene (supD) downstream of J23108 Anderson promoter. When co-transformed, this part allows the expression of a protein coding gene with up to 5 serine codons substituted for amber stop codons (TAG) - such as BBa_K2725012 - P1003 Kanamycin resistance gene with 5 amber substitutions. | Serine amber suppressor tRNA gene (supD) downstream of J23108 Anderson promoter. When co-transformed, this part allows the expression of a protein coding gene with up to 5 serine codons substituted for amber stop codons (TAG) - such as BBa_K2725012 - P1003 Kanamycin resistance gene with 5 amber substitutions. | ||
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Revision as of 22:10, 17 October 2018
J23108 - supD - B1006
Serine amber suppressor tRNA gene (supD) downstream of J23108 Anderson promoter. When co-transformed, this part allows the expression of a protein coding gene with up to 5 serine codons substituted for amber stop codons (TAG) - such as BBa_K2725012 - P1003 Kanamycin resistance gene with 5 amber substitutions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 77
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 80