Difference between revisions of "Part:BBa K2765050"

Revision as of 22:07, 17 October 2018

Complete roGFP2-Orp1 expression cassette started by the promoter TEF2

This part consists of the promoter TEF2p , redox sensor roGFP2-Orp1 and the terminator CYC1t. The promoter TEF2p is an constitutive promoter from Saccharomyces cerevisiae CEN.PK-1c（see the details in part：BBa_K124001）. roGFP2-Orp1 is a fusion protein which can response to the redox status in the cell rapidly as well as sensitively(see the details in part:BBa_K2765043).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1252
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1564

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2765050 short</partinfo>
-This part consists of the promoter TEF2p , redox sensor roGFP2-Orp1 and the terminator cyc1. The promoter TEF2p is an constitutive promoter from <i>Saccharomyces cerevisiae CEN.PK-1c</i>（see the details in part：BBa_K124001）. roGFP2-Orp1 is a fusion protein which can response to the redox status in the cell rapidly as well as sensitively(see the details in part:BBa_K2765043).
+This part consists of the promoter TEF2p , redox sensor roGFP2-Orp1 and the terminator CYC1t. The promoter TEF2p is an constitutive promoter from <i>Saccharomyces cerevisiae</i> CEN.PK-1c（see the details in part：BBa_K124001）. roGFP2-Orp1 is a fusion protein which can response to the redox status in the cell rapidly as well as sensitively(see the details in part:BBa_K2765043).
 [[Image: T--BIT-China--iGEM2018-PartsOutput-TEF2ligated.png |center|400px|]]