Difference between revisions of "Part:BBa K2717016:Experience"
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===Applications of BBa_K2717016=== | ===Applications of BBa_K2717016=== | ||
| − | The whole pathway can be described in Figure | + | The whole pathway can be described in Figure 19. |
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| + | https://static.igem.org/mediawiki/2018/b/ba/T--BNU-China--_R19.png | ||
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| + | Fig.19 Plasmids constructed for testing TGATG efficiency. | ||
| + | This part is the Control group 2. | ||
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Considering the mutual influence between two kinds of fluorescence, we design this part to accurately test the efficiency of stop-start codon "TGATG". Flow Cytometer (FCM) was used to measure the bacteria's number and a relative fluorescence. As expectation, both green and pink fluorescence can be detected in some bacteria. Then, we compared the final relative fluorescent intensity with that of TGATG-experimental group （BBa_K2717008）and calculated the efficiency. The specific experiments and data processing can be found in our wiki. | Considering the mutual influence between two kinds of fluorescence, we design this part to accurately test the efficiency of stop-start codon "TGATG". Flow Cytometer (FCM) was used to measure the bacteria's number and a relative fluorescence. As expectation, both green and pink fluorescence can be detected in some bacteria. Then, we compared the final relative fluorescent intensity with that of TGATG-experimental group （BBa_K2717008）and calculated the efficiency. The specific experiments and data processing can be found in our wiki. | ||
Latest revision as of 21:41, 17 October 2018
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Applications of BBa_K2717016
The whole pathway can be described in Figure 19.
Fig.19 Plasmids constructed for testing TGATG efficiency. This part is the Control group 2.
Considering the mutual influence between two kinds of fluorescence, we design this part to accurately test the efficiency of stop-start codon "TGATG". Flow Cytometer (FCM) was used to measure the bacteria's number and a relative fluorescence. As expectation, both green and pink fluorescence can be detected in some bacteria. Then, we compared the final relative fluorescent intensity with that of TGATG-experimental group （BBa_K2717008）and calculated the efficiency. The specific experiments and data processing can be found in our wiki.
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