Difference between revisions of "Part:BBa K2680051"
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37C Temperature-Sensitive mScarlet-I pdt#3. Components: J23107 ts-CI + R0051 mScarlet-I pdt#3 | 37C Temperature-Sensitive mScarlet-I pdt#3. Components: J23107 ts-CI + R0051 mScarlet-I pdt#3 | ||
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+ | In this circuit, temperature-sensitive CI is expressed constitutively by J23107, a medium-weak constitutive promoter. This temperature-sensitive CI then represses the downstream CI promoter. Once the temperature is sufficiently raised, the ts-CI protein denatures, loosing its repression capabilities. Therefore, the CI promoter is freed, allowing for expression of the rapidly-maturing mScarlet-I. The mScarlet-I in this circuit has a pdt, a protein degradation tag, allowing for degradation by mf-Lon protease (not included in this circuit). Specifically, this circuit contains pdt#3, a strong degradation tag from the part series K2333401-K2333406 or K2333401. | ||
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Revision as of 21:36, 17 October 2018
37C Temperature Sensitive mScarlet-I pdt#3
37C Temperature-Sensitive mScarlet-I pdt#3. Components: J23107 ts-CI + R0051 mScarlet-I pdt#3
In this circuit, temperature-sensitive CI is expressed constitutively by J23107, a medium-weak constitutive promoter. This temperature-sensitive CI then represses the downstream CI promoter. Once the temperature is sufficiently raised, the ts-CI protein denatures, loosing its repression capabilities. Therefore, the CI promoter is freed, allowing for expression of the rapidly-maturing mScarlet-I. The mScarlet-I in this circuit has a pdt, a protein degradation tag, allowing for degradation by mf-Lon protease (not included in this circuit). Specifically, this circuit contains pdt#3, a strong degradation tag from the part series K2333401-K2333406 or K2333401.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 51
Illegal NheI site found at 74
Illegal NotI site found at 1518 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]