Difference between revisions of "Part:BBa K2656122"
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| − | <li></html>[https://parts.igem.org/Part:BBa_K2656003 BBa_K2656003]: the [https://parts.igem.org/Part: BBa_R0062 BBa_R0062] plux inducible promoter in its Golden Braid compatible version. <html></li> | + | <li></html>[https://parts.igem.org/Part:BBa_K2656003 BBa_K2656003]: the [https://parts.igem.org/Part:BBa_R0062 BBa_R0062] plux inducible promoter in its Golden Braid compatible version. <html></li> |
<li></html>[https://parts.igem.org/Part:BBa_BBa_K2656011 BBa_K2656011]: the [https://parts.igem.org/Part:BBa_B0034 B0034] ribosome biding site in its Golden Braid compatible version <html></li> | <li></html>[https://parts.igem.org/Part:BBa_BBa_K2656011 BBa_K2656011]: the [https://parts.igem.org/Part:BBa_B0034 B0034] ribosome biding site in its Golden Braid compatible version <html></li> | ||
<li></html> [https://parts.igem.org/Part:BBa_K2656022 BBa_K2656022]: the [https://parts.igem.org/Part:BBa_K2656022 BBa_K2656022] GFPmut3b sequence in its Golden Braid standardized version <html></li> | <li></html> [https://parts.igem.org/Part:BBa_K2656022 BBa_K2656022]: the [https://parts.igem.org/Part:BBa_K2656022 BBa_K2656022] GFPmut3b sequence in its Golden Braid standardized version <html></li> | ||
Latest revision as of 21:33, 17 October 2018
pLux-GFPmut3b transcriptional unit
Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1).
This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:
- BBa_K2656003: the BBa_R0062 plux inducible promoter in its Golden Braid compatible version.
- BBa_K2656011: the B0034 ribosome biding site in its Golden Braid compatible version
- BBa_K2656022: the BBa_K2656022 GFPmut3b sequence in its Golden Braid standardized version
- Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]