Difference between revisions of "Part:BBa K2843002"
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<partinfo>BBa_K2843002 short</partinfo> | <partinfo>BBa_K2843002 short</partinfo> | ||
| − | This is BBa_K2843000 operon with the "Fluoride Binding Mutant", which is a mutant of the fluoride | + | This is BBa_K2843000 operon with the "Fluoride Binding Mutant", which is a mutant of the fluoride riboswitch. This serves as a controlled experiment because if there is growth of delta-CRCB Ecoli on the chloramphenicol plates than this indicates that growth of delta-CRCB is not entirely dependent only on the presence of fluoride. |
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Latest revision as of 21:32, 17 October 2018
Fluoride RBS Mutant
This is BBa_K2843000 operon with the "Fluoride Binding Mutant", which is a mutant of the fluoride riboswitch. This serves as a controlled experiment because if there is growth of delta-CRCB Ecoli on the chloramphenicol plates than this indicates that growth of delta-CRCB is not entirely dependent only on the presence of fluoride.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 47
Illegal NheI site found at 70 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 148
Illegal XhoI site found at 189 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]