Difference between revisions of "Part:BBa K2684005"
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Revision as of 21:27, 17 October 2018
SpyCatcher-CotA
SpyCatcher fused with CotA by 2xGGGGS linker
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 514
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1903
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 841
This part could be used to produce CotA laccase fused with SpyCatcher (BBa_K2684005). Team SHSBNU_China designed this part to improve the the enzyme activity of traditional CotA. When applying the gene CotA without SpyCatcher, the laccase cannot be suspended onto the biofilm created by CsgA-Spytag. Therefore, there will not be significant improvement in enzyme activity. However, while applying with CsgA-Spytag, the enzyme activity dramatically increased, as shown in the graph below.
