Difference between revisions of "Part:BBa K2719003:Experience"
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===Applications of BBa_K2719003=== | ===Applications of BBa_K2719003=== | ||
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| + | <p>This part was cloned on pSB1C3 by restriction with EcoRI and PstI and transformed into <i>E.coli</i> DH5a, obtaining white colonies (see figure 1).</p> | ||
| + | [[file:T--TecCEM--OPGSTColonies.png|500px]] | ||
| + | <p><i>Figure 1.</i> Colonies transformed with optimized GST.</p> | ||
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| + | <p>Latter it has extracted and run on an agarose gel electrophoresis (see figure 2).</p> | ||
| + | [[file:T--TecCEM--GelK2719003.png|500px]] | ||
| + | <p><i>Figure 2.</i> 0.85% agarose gel with GelRed, MW)NEB 1Kb DNA Ladder, 1)Extraction GST Optimized (BBa_K2719003), 2)Extraction GST Optimized (BBa_K2719003) duplicate.</p> | ||
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| + | <p>Finally it was treated with NotI and run on an agarose gel electrophoresis to prove the presence of insert (see figure 3).</p> | ||
| + | [[file:T--TecCEM--ResK2719003.png|500px]] | ||
| + | <p><i>Figure 3.</i> 0.85% agarose gel with GelRed, MW)NEB 1Kb DNA Ladder, 1)Restriction GST Optimized with NotI (BBa_K2719003), 2)Restriction GST Optimized with Not I (BBa_K2719003) duplicate.</p> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 21:24, 17 October 2018
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K2719003
This part was cloned on pSB1C3 by restriction with EcoRI and PstI and transformed into E.coli DH5a, obtaining white colonies (see figure 1).
Figure 1. Colonies transformed with optimized GST.
Latter it has extracted and run on an agarose gel electrophoresis (see figure 2).
Figure 2. 0.85% agarose gel with GelRed, MW)NEB 1Kb DNA Ladder, 1)Extraction GST Optimized (BBa_K2719003), 2)Extraction GST Optimized (BBa_K2719003) duplicate.
Finally it was treated with NotI and run on an agarose gel electrophoresis to prove the presence of insert (see figure 3).
Figure 3. 0.85% agarose gel with GelRed, MW)NEB 1Kb DNA Ladder, 1)Restriction GST Optimized with NotI (BBa_K2719003), 2)Restriction GST Optimized with Not I (BBa_K2719003) duplicate.
User Reviews
UNIQ20cc706d4d41873d-partinfo-00000000-QINU UNIQ20cc706d4d41873d-partinfo-00000001-QINU