Difference between revisions of "Part:BBa K2587005"
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<partinfo>BBa_K2587005 short</partinfo>
 
<partinfo>BBa_K2587005 short</partinfo>
  
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LasR is the regulator of the quorum sensing system from <i>Pseudomonas aeruginosa</i>. In this case the sequence is codon optimised for <i>E. coli</i> and together with LasI and P<i><sub>las</sub></i> is supposed to induce expression of a gene of interest in a population density dependent manner.
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LasR is the regulator of the quorum sensing system from <i>Pseudomonas aeruginosa</i>. In this case the sequence is codon optimized for <i>E. coli</i>. It works as a repressor for P<i><sub>las</sub></i>; upon binding of the corresponding quorum sensing molecule, the promoter is released and is able to induce expression of a gene of interest in a population density dependent manner.
  
 
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Revision as of 21:04, 17 October 2018


lasR

LasR is the regulator of the quorum sensing system from Pseudomonas aeruginosa. In this case the sequence is codon optimized for E. coli. It works as a repressor for Plas; upon binding of the corresponding quorum sensing molecule, the promoter is released and is able to induce expression of a gene of interest in a population density dependent manner.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 579
    Illegal SpeI site found at 738
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 579
    Illegal SpeI site found at 738
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 579
    Illegal SpeI site found at 738
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 579
    Illegal SpeI site found at 738
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 731