Difference between revisions of "Part:BBa K2624000:Experience"
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To improve the selectivity of our therapy system, we utilize hTERT, the cancer-specific promoter, and HULC, the HCC-specific promoter, to open an AND-gated system to target HCC (Hepatocellular carcinoma). pGL3-Basic vector, a promoterless vector is used for measuring the activity of promoter with a luciferase assay. We determined the activity of the two promoters by it. Experimental results show that the promoter has a good startup efficiency,although the efficiency is lower than CMV promoter. | To improve the selectivity of our therapy system, we utilize hTERT, the cancer-specific promoter, and HULC, the HCC-specific promoter, to open an AND-gated system to target HCC (Hepatocellular carcinoma). pGL3-Basic vector, a promoterless vector is used for measuring the activity of promoter with a luciferase assay. We determined the activity of the two promoters by it. Experimental results show that the promoter has a good startup efficiency,although the efficiency is lower than CMV promoter. | ||
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| + | Figure. The activity of the promoters | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 20:54, 17 October 2018
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Applications of BBa_K2624000
To improve the selectivity of our therapy system, we utilize hTERT, the cancer-specific promoter, and HULC, the HCC-specific promoter, to open an AND-gated system to target HCC (Hepatocellular carcinoma). pGL3-Basic vector, a promoterless vector is used for measuring the activity of promoter with a luciferase assay. We determined the activity of the two promoters by it. Experimental results show that the promoter has a good startup efficiency,although the efficiency is lower than CMV promoter.
Figure. The activity of the promoters
User Reviews
UNIQ703ed7e5122867f1-partinfo-00000000-QINU UNIQ703ed7e5122867f1-partinfo-00000001-QINU