Difference between revisions of "Part:BBa K2685006"
 
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<partinfo>BBa_K2685006 short</partinfo>
 
<partinfo>BBa_K2685006 short</partinfo>
  
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     This part expresses an efflux pump protein that selectively pumps tetracycline from the cell with Mg + isomeric tetracycline to restrict tetracycline entry into the cell ribosome, thereby enhancing the tolerance of E. coli to tetracycline by inserting E. coli containing the psb1c3 empty plasmid and our E. coli with this part in the absence of The ABS value was measured under the same concentration of tetracycline. It can be seen that E. coli containing only psb1c3 empty plasmid has little tolerance to tetracycline, and we added this part E.coli has certain tolerance to tetracycline.  
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     <p>This parts can provide tetracycline tolerance to our constructive detecting chasis which including tetA and tetO genes.</p>
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<p>  TetA encodes the tetracycline efflux pump protein, which selectively transports tetracycline from thecytoplasm to the periplasm in exchange for protons, thus limiting tetracycline's access to ribosomes within cells.They are proton power dependent, single polypeptide, drug-specific efflux pump, and it belong to the main promoting factor superfamily.</p>
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<p>  We also design some single sites mutation comparing with the original sequence that we have got from the biobrick bank.</p>
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                    <div class="at-user__avatar" align="center">
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"https://static.igem.org/mediawiki/parts/e/e6/T--TUST_China--part_document_072.png"
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                    </div>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 20:40, 17 October 2018


TetO+TetA

This parts can provide tetracycline tolerance to our constructive detecting chasis which including tetA and tetO genes.

TetA encodes the tetracycline efflux pump protein, which selectively transports tetracycline from thecytoplasm to the periplasm in exchange for protons, thus limiting tetracycline's access to ribosomes within cells.They are proton power dependent, single polypeptide, drug-specific efflux pump, and it belong to the main promoting factor superfamily.

We also design some single sites mutation comparing with the original sequence that we have got from the biobrick bank.

"T--TUST_China--part_document_072.png"

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 897
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 343
    Illegal NgoMIV site found at 503
    Illegal NgoMIV site found at 871
  • 1000
    COMPATIBLE WITH RFC[1000]