Difference between revisions of "Part:BBa K2596005:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We codon optimized cscB for our cyanobacterial chassis. However, after optimization, there was an illegal PstI cut site. We were able to remove the cut site through a silent point mutation. The codon that we replaced it with was fortunately equally as abundant as the prior codon. | + | We codon optimized <i>cscB</I> for our cyanobacterial chassis. However, after optimization, there was an illegal PstI cut site. We were able to remove the cut site through a silent point mutation. The codon that we replaced it with was fortunately equally as abundant as the prior codon. |
===Source=== | ===Source=== | ||
| − | E. coli | + | <i>E. coli</i> |
===References=== | ===References=== | ||
Latest revision as of 20:23, 17 October 2018
cscB (sucrose permease) from E. coli
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 481
Illegal NgoMIV site found at 604 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We codon optimized cscB for our cyanobacterial chassis. However, after optimization, there was an illegal PstI cut site. We were able to remove the cut site through a silent point mutation. The codon that we replaced it with was fortunately equally as abundant as the prior codon.
Source
E. coli
References
Qiao C, Duan Y, Zhang M, et al. Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942. Appl Environ Microbiol. 2018;84(2): e02023-17. doi: 10.1128/AEM.02023-17.
McEwen JT, Machado IMP, Connor MR, et al. Engineering Synechococcus elongatus PCC 7942 for Continuous Growth under Diurnal Conditions. Appl Environ Microbiol. 2013;79(5): 1668-1675. doi: 10.1128/AEM.03326-12.