Difference between revisions of "Part:BBa K2719004:Experience"

Revision as of 20:15, 17 October 2018

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2719004

This part was optimized Escherichia coli BL21 (DE3) so it could be transformed into it and also cloned in pSB1C3. For proving it presence it was necessary to make two agarose gels, one for the entire plasmid (figure 2) and the other one for the restriction part in order to observe the insert. (Figure 3)

Figure 1. Colonies transformed with Tenascin Fibronectin domains.

Figure 2. 0.85% agarose gel with GelRed, MW)NEB 1Kb Plus DNA Ladder, 1)Extraction Tenascin 5 Fibronectin domains, 2)Extraction Tenascin 5 Fibronectin domains duplicate.

User Reviews

UNIQ41c3487fd0957482-partinfo-00000000-QINU UNIQ41c3487fd0957482-partinfo-00000001-QINU

@@ Line 5: / Line 5: @@
 ===Applications of BBa_K2719004===
-<p>This part was optimized <i>Escherichia coli</i> BL21 (DE3) so it could be transformed into it and also cloned in pSB1C3. For proving it presence it was necessary to make two agarose gels, one for the entire plasmid and the other one for the restriction part in order to observe the insert. (Figure 2)</p>
+<p>This part was optimized <i>Escherichia coli</i> BL21 (DE3) so it could be transformed into it and also cloned in pSB1C3. For proving it presence it was necessary to make two agarose gels, one for the entire plasmid (figure 2) and the other one for the restriction part in order to observe the insert. (Figure 3)</p>
 [[file:T--TecCEM--TCSD5Colonies.png|500px]]
 <p><i>Figure 1.</i> Colonies transformed with Tenascin Fibronectin domains.</p>
+[[file:T--TecCEM--GelK2719004.png|500px]]
+<p><i>Figure 2.</i> 0.85% agarose gel with GelRed, MW)NEB 1Kb Plus DNA Ladder, 1)Extraction Tenascin 5 Fibronectin domains, 2)Extraction Tenascin 5 Fibronectin domains duplicate.</p>
 ===User Reviews===