Difference between revisions of "Part:BBa K2685016:Design"

 
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<partinfo>BBa_K2685016 short</partinfo>
 
<partinfo>BBa_K2685016 short</partinfo>
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<div class="at-user__avatar" align="center">
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"https://static.igem.org/mediawiki/parts/9/99/Parts_document_2.png"
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                    </div>
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<p>This part is the best of the many degradation circuits we have built. It is made up of Lip and suicide switch.We cocultured </p>
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<p> BBa_K2685007 and BBa_K2685016 at different concentrations of tetracycline. Lip degradation effect was measured by</p>
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<p> using unit fluorescence intensity because Lip degraded tetracycline caused BBa_K2685007 concentration growth thereby </p>
 +
<p> increased its unit production fluorescence intensity. From the pictures we can see that the unit fluorescence intensity </p>
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<p> of BBa_K2685007 mixed with BBa_K2685016 is generally higher than that of BBa_K2685007 alone.This further indicates that </p>
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<p> Lip can efficiently degrade tetracycline in the environment.</p>
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<p>The figure below shows the common validation of the different degradation circuits we built. Compared with the MNP and</p>
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<p>bi-enzyme degradation circuits, BBa_K2685016 is one of the parts with the best degradation effect.</p>
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<partinfo>BBa_K2685016 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2685016 SequenceAndFeatures</partinfo>

Revision as of 20:10, 17 October 2018


Degradation Part (ptet+Lip+LacI+plac+phi174E)

"Parts_document_2.png"

This part is the best of the many degradation circuits we have built. It is made up of Lip and suicide switch.We cocultured

BBa_K2685007 and BBa_K2685016 at different concentrations of tetracycline. Lip degradation effect was measured by

using unit fluorescence intensity because Lip degraded tetracycline caused BBa_K2685007 concentration growth thereby

increased its unit production fluorescence intensity. From the pictures we can see that the unit fluorescence intensity

of BBa_K2685007 mixed with BBa_K2685016 is generally higher than that of BBa_K2685007 alone.This further indicates that

Lip can efficiently degrade tetracycline in the environment.

The figure below shows the common validation of the different degradation circuits we built. Compared with the MNP and

bi-enzyme degradation circuits, BBa_K2685016 is one of the parts with the best degradation effect.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2355
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 565
    Illegal AgeI site found at 542
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

oxidize the tetracycline


Source

constructed by our team

References