Difference between revisions of "Part:BBa K2623009"

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===pKaiBC in pathways===
 
===pKaiBC in pathways===
 
<table><tr><th>
 
<table><tr><th>
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[[Image:PKaiBC 1 .gif ‎|thumb|500px|Fig.1 Simplified model of the central circadian oscillator and output activities.]]</tr><th>
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[[Image:PKaiBC 1 .gif ‎|thumb|500px|Fig.1 Simplified model of the central circadian oscillator and output activities.[1]]]</th><th></table>
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[[Image:PKaiBC 2 .gif ‎|thumb|500px|Fig.2 PKaiBC is one of the specific promoters that can be activated by RpaA-p.]]</th><th></table>
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<table><tr><th>[[Image:PKaiBC 2 .gif ‎|thumb|400px|Fig.2 PKaiBC is one of the specific promoters that can be activated by RpaA-p.[2]]]</th><th></table>
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===Reference===
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[1]Paddock, Mark, Boyd, et al. Active Output State of the Synechococcus Kai Circadian Oscillator[J]. Pnas, 2014, 110(40):E3849-E3857.<br>
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[2]Espinosa J, Boyd J S, Cantos R, et al. Cross-talk and regulatory interactions between the essential response regulator RpaB and cyanobacterial circadian clock output[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(7):2198-203.<br>
  
 
More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results <br>
 
More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results <br>

Latest revision as of 19:59, 17 October 2018


pKaiBC promoter

Summary

As a component of the Kai circuit, pKaiBC promoter can be specific recognize and active by the phosphorylated RpaA，It participates in the construction of biological clock，as an important part of circadian rhythm-related protein expression in cyanobacteria.
The fragment is 89 bp.

pKaiBC in pathways

Fig.1 Simplified model of the central circadian oscillator and output activities.[1]
Fig.2 PKaiBC is one of the specific promoters that can be activated by RpaA-p.[2]

Reference

[1]Paddock, Mark, Boyd, et al. Active Output State of the Synechococcus Kai Circadian Oscillator[J]. Pnas, 2014, 110(40):E3849-E3857.
[2]Espinosa J, Boyd J S, Cantos R, et al. Cross-talk and regulatory interactions between the essential response regulator RpaB and cyanobacterial circadian clock output[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(7):2198-203.

More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]