Difference between revisions of "Part:BBa K2765051"

 
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This part is the expression cassette of the roGFP2-Orp1 .We completed roGFP2-Orp1 expression cassette started by the promoter TEF1.
 
This part is the expression cassette of the roGFP2-Orp1 .We completed roGFP2-Orp1 expression cassette started by the promoter TEF1.
 
It can express green fluorescent protein roGFP2-Orp1，and this protein can reflect the accumulation of ROS in cells.
 
It can express green fluorescent protein roGFP2-Orp1，and this protein can reflect the accumulation of ROS in cells.
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[[Image: T--BIT-China--iGEM2018-PartsOutput-TEF1geneline.png |center|400px|]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:54, 17 October 2018


Complete roGFP2-Orp1 expression cassette started by the promoter TEF1

This part is the expression cassette of the roGFP2-Orp1 .We completed roGFP2-Orp1 expression cassette started by the promoter TEF1. It can express green fluorescent protein roGFP2-Orp1，and this protein can reflect the accumulation of ROS in cells.

T--BIT-China--iGEM2018-PartsOutput-TEF1geneline.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1251
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 157
    Illegal SapI.rc site found at 1563