Difference between revisions of "Part:BBa K2277000"

 
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===Functional Parameters===
 
===Functional Parameters===
 
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Revision as of 19:44, 17 October 2018


A gene expresses one kind of lyase which can crack the cell wall of E.coli cells.

Lysin is a protein-coding gene. It can express a kind of lyase. This kind of lyase can crack the cell wall of E.coli cells so that intercellular products can be released to the broth. The lyase works from inside the E.coli cells and will not be released before the cell lysis. Its lysis rate can be up to 99.8% in pET28a(+) under IPTG induction. This lyase can absolutely simplify the cells' disruption process in fermentation industry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 555
  • 1000
    COMPATIBLE WITH RFC[1000]

sss