Difference between revisions of "Part:BBa K2627004:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K2627004 short</partinfo>
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<partinfo>BBa_K2627003 short</partinfo>
  
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<partinfo>BBa_K2627004 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2627003 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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We managed to delete the restriction site for EcoRI through Gibson Assembly.
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crRNA specifically targeting gltA gene and repress gene expression through type I-E CRISPR system
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===Source===
 
===Source===
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crRNA originated in Escherichia coli EE-E15
  
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===References===
 
Chang, Y., et al., Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system. Microbial Cell Factories, 2016. 15(1).
 
Chang, Y., et al., Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system. Microbial Cell Factories, 2016. 15(1).
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===References===
 

Latest revision as of 19:23, 17 October 2018


crRNA targeting GltA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

crRNA specifically targeting gltA gene and repress gene expression through type I-E CRISPR system

Source

crRNA originated in Escherichia coli EE-E15

References

Chang, Y., et al., Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system. Microbial Cell Factories, 2016. 15(1).