Difference between revisions of "Part:BBa K2569031"

 
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Expressing this BioBrick in E. coli enables the bacterium to degrade nicotine. Nicotine is one of the most harmful ingredients in cigarette and is the biggest resistance to smoking cessation. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in E. coli can convert nicotine to pseudo-oxidation can be achieved by resting cells of E. coli as a catalyst in a whole-cell reaction.
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Expressing this BioBrick in <i>Pseudomonas fluorescence</i>-pf5 cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in <i>P.fluorescence</i>-pf5 can convert nicotine to pseudo-oxidation can be achieved by resting cells of <i>P.fluorescence</i>-pf5 as a catalyst in a whole-cell reaction.So, with our strong promoper PdnaA enhancing, NicA2 is overexpressed and survived in high concentration of nicotin. And nicotin can get fully degradated.
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===Nicotine Degradation Pathway===
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<span class="image fit">
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  https://static.igem.org/mediawiki/2018/7/70/T--SKLMT-China--nicotine-pathway.png
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  Fig. Nicotin degradation pathway.
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===Usage and Biology===
 
===Usage and Biology===
  

Latest revision as of 19:02, 17 October 2018


PdnaA -NicA2

Expressing this BioBrick in Pseudomonas fluorescence-pf5 cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in P.fluorescence-pf5 can convert nicotine to pseudo-oxidation can be achieved by resting cells of P.fluorescence-pf5 as a catalyst in a whole-cell reaction.So, with our strong promoper PdnaA enhancing, NicA2 is overexpressed and survived in high concentration of nicotin. And nicotin can get fully degradated.

Nicotine Degradation Pathway

  T--SKLMT-China--nicotine-pathway.png
  Fig. Nicotin degradation pathway.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1393
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]