Difference between revisions of "Part:BBa K2847002"

Revision as of 18:50, 17 October 2018

pSC101 ori + RepA (thermosensitive mutant) + AmpR gene

pSC101 ori + RepA (thermosensitive mutant) + AmpR gene amplified from vector pE-FLP (Addgene Plasmid #45978)

NB! This part is NOT BioBrick compatible! It is designed to be used as a PCR matrix for the amplification of the pSC101* thermosensitive origin of replication. The cheapest and fastest way to put it in a pSB1-based biobrick vector is to use Aqua cloning - see the Main page of part BBa_K2515002 for more details.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SpeI site found at 1163
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SpeI site found at 1163
Illegal NotI site found at 1482
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SpeI site found at 1163
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SpeI site found at 1163
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2128

@@ Line 4: / Line 4: @@
 pSC101 ori + RepA (thermosensitive mutant) + AmpR gene amplified from vector pE-FLP (Addgene Plasmid #45978)
+<b>NB! This part is NOT BioBrick compatible!</b> It is designed to be used as a PCR matrix for the amplification of the pSC101* thermosensitive origin of replication. The cheapest and fastest way to put it in a pSB1-based biobrick vector is to use Aqua cloning - see the Main page of part <partinfo>BBa_K2515002</partinfo> for more details.
 <!-- Add more about the biology of this part here