Difference between revisions of "Part:BBa K390501:Experience"

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Available Phasin biobrick (BBa_K208001) are found in Lac promoter-Phasin-HlyA biobrick, BBa_K390501. Through sequencing, it was found to contain a stop codon. A set of PCR primers was designed and used to remove the stop codon. In the primer design, a HindIII restriction enzyme site, which is not present in the original biobrick, was introduced and allow the confirmation of the success stop codon removal.
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https://static.igem.org/mediawiki/parts/b/b0/T--Edinburgh_OG_BBa_K2739007--image_5.jpeg
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Figure 1. The sequence alignment of BBa_K390501, LacP-Phasin-Hly-VR-170918-08-27(The sequencing result of BBa K2739007) and original-phasinHlyA-VR-170918-08-28 (The sequencing result of BBa_K390501) via Benchling. The selected (blue) part of the sequence indicated the stop codon.
 
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Revision as of 18:17, 17 October 2018

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K390501

This part is used for phasin secretion. The same general structure of this composite part could be used to for the secretion of other proteins by substituting phasin for another part of interest. These would need to be made Silver-fusion compatible.

User Reviews

UNIQ9f9c8bbbc239168b-partinfo-00000000-QINU UNIQ9f9c8bbbc239168b-partinfo-00000001-QINU

No review score entered. Username

Enter the review inofrmation here. Available Phasin biobrick (BBa_K208001) are found in Lac promoter-Phasin-HlyA biobrick, BBa_K390501. Through sequencing, it was found to contain a stop codon. A set of PCR primers was designed and used to remove the stop codon. In the primer design, a HindIII restriction enzyme site, which is not present in the original biobrick, was introduced and allow the confirmation of the success stop codon removal.

T--Edinburgh_OG_BBa_K2739007--image_5.jpeg Figure 1. The sequence alignment of BBa_K390501, LacP-Phasin-Hly-VR-170918-08-27(The sequencing result of BBa K2739007) and original-phasinHlyA-VR-170918-08-28 (The sequencing result of BBa_K390501) via Benchling. The selected (blue) part of the sequence indicated the stop codon.

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UNIQ9f9c8bbbc239168b-partinfo-00000003-QINU