Difference between revisions of "Part:BBa K2888000"

 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2888000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2888000 SequenceAndFeatures</partinfo>
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===Application===
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We intend to use lysozyme gene to lyse cyanoabacteria which has unusually thick and complex cell wall. The cyanobacterium has a much thicker peptidoglycan layer and a unique external layer containing an S-layer and exopolysaccharide. In our experiment, we easily mix Bugbuster and expressed protein in order to fully lyse cyanobacteria cell wall
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4478636/bin/13036_2015_7_Fig1_HTML.jpg
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Revision as of 18:01, 17 October 2018


cyanophage lysozyme gene

This cyanophage lysozyme gene can target to and specifically lyse the cell wall of cyanobacteria by cooperating with Bugbuster chemical solution. Among the 27 lysozyme gene family, this cyanophage lysozyme gene gives the most efficient and effective soluble expression as well as purification. Unlike other bacteria, cyanobacteria have much thicker layers which are difficult to be lysed with common enzymes. Therefore, cyanobacteria-targeted lysozyme and other chemicals breaking through these unique barriers are needed in chemical lysis process.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Application

We intend to use lysozyme gene to lyse cyanoabacteria which has unusually thick and complex cell wall. The cyanobacterium has a much thicker peptidoglycan layer and a unique external layer containing an S-layer and exopolysaccharide. In our experiment, we easily mix Bugbuster and expressed protein in order to fully lyse cyanobacteria cell wall

13036_2015_7_Fig1_HTML.jpg