Difference between revisions of "Part:BBa K2533049:Design"

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===Design Notes===
 
===Design Notes===
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Compared with cyanobacteria, we find Rhodopseudomonas palustris is more suitable than cyanobacteria to provide Lactate to Shewenella due to its charactestics of Anaerobic respiration.Therefore, we decided to modify Rhodopseudomonas palustris so that it could produce lactate under the anaerobic condition and transport lactate to the extracellular. And we have found that if we want to enhance the production of lactate from Rhodopseudomonas palustris, we can promote the convertion efficiency of pyruvate to D-lactate and malate to L-lactate. Therefore, we decided to use this two genes, mleS and ldhA to help Rhodopseudomonas palustris to produce lactate. Also, Considering the necessity of helping Rhodopseudomonas palustris to transport lactate out of the membrane, we need to add a lldP gene.
+
Compared with cyanobacteria, we find Rhodopseudomonas palustris is more suitable than cyanobacteria to provide Lactate for Shewenella due to its charactestics of Anaerobic respiration.Therefore, we decided to modify Rhodopseudomonas palustris so that it could produce lactate under anaerobic condition and transport lactate to the extracellular. And we have found that if we want to enhance the production of lactate from Rhodopseudomonas palustris, we can promote the convertion efficiency of pyruvate to D-lactate and malate to L-lactate. Therefore, we decided to use this two genes, mleS and ldhA, to help Rhodopseudomonas palustris to produce lactate. Also, considering the necessity of helping Rhodopseudomonas palustris transport lactate out of the membrane, we need to add a lldP gene.
  
 
===Source===
 
===Source===
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===References===
 
===References===
 +
【1】：Henan journal of animal husbandry and veterinary medicine，by Wang yue xian ; Liu de hai
 +
【2】：The Capacity of Photosynthesis Bacteria Rhodopseudomonas palustris Assimilating to Phosphate,  Bulletin of Science and Technology,  2002,  issue 2, pp 142-146
 +
【3】：KEGG, https://www.genome.jp/kegg/pathway.html
 +
【4】：Cloning and sequence analysis of the gene encoding Lactococcus lactis malolactic enzyme: relationships with malic enzymes FEMS Microbiol Lett. 1994 Feb 1;116(1):79-86
 +
【5】：Fine tuning the transcription of ldhA for d-lactate production August 2012, Volume 39, Issue 8, pp 1209–1217
 +
【6】：Transport of L-Lactate, D-Lactate, and Glycolate by the LldP and GlcA Membrane Carriers of Escherichia coli Volume 290, Issue 2, 18 January 2002, Pages 824-829
 +
【7】：Enhancement of Hydrogen Production and Carbon Fixation in Purple Nonsulfur Bacterium Bacterium by Synthetic Biology  Shou-Chen Lo
 +
【8】：Jcat, http://www.jcat.de/#opennewwindow

Latest revision as of 17:17, 17 October 2018


RBS-lldP-RBS-ldhA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 293
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 49
    Illegal NgoMIV site found at 415
    Illegal NgoMIV site found at 487
    Illegal NgoMIV site found at 556
    Illegal NgoMIV site found at 690
    Illegal NgoMIV site found at 706
    Illegal NgoMIV site found at 904
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 786
    Illegal BsaI.rc site found at 682
    Illegal BsaI.rc site found at 2664


Design Notes

Compared with cyanobacteria, we find Rhodopseudomonas palustris is more suitable than cyanobacteria to provide Lactate for Shewenella due to its charactestics of Anaerobic respiration.Therefore, we decided to modify Rhodopseudomonas palustris so that it could produce lactate under anaerobic condition and transport lactate to the extracellular. And we have found that if we want to enhance the production of lactate from Rhodopseudomonas palustris, we can promote the convertion efficiency of pyruvate to D-lactate and malate to L-lactate. Therefore, we decided to use this two genes, mleS and ldhA, to help Rhodopseudomonas palustris to produce lactate. Also, considering the necessity of helping Rhodopseudomonas palustris transport lactate out of the membrane, we need to add a lldP gene.

Source

Escherichia coli str. K-12 substr. MG1655 & Escherichia coli str. K-12 substr. MG1655

References

【1】：Henan journal of animal husbandry and veterinary medicine，by Wang yue xian ; Liu de hai 【2】：The Capacity of Photosynthesis Bacteria Rhodopseudomonas palustris Assimilating to Phosphate, Bulletin of Science and Technology, 2002, issue 2, pp 142-146 【3】：KEGG, https://www.genome.jp/kegg/pathway.html 【4】：Cloning and sequence analysis of the gene encoding Lactococcus lactis malolactic enzyme: relationships with malic enzymes FEMS Microbiol Lett. 1994 Feb 1;116(1):79-86 【5】：Fine tuning the transcription of ldhA for d-lactate production August 2012, Volume 39, Issue 8, pp 1209–1217 【6】：Transport of L-Lactate, D-Lactate, and Glycolate by the LldP and GlcA Membrane Carriers of Escherichia coli Volume 290, Issue 2, 18 January 2002, Pages 824-829 【7】：Enhancement of Hydrogen Production and Carbon Fixation in Purple Nonsulfur Bacterium Bacterium by Synthetic Biology Shou-Chen Lo 【8】：Jcat, http://www.jcat.de/#opennewwindow