Difference between revisions of "Part:BBa K2533055"
| Line 6: | Line 6: | ||
<h1>'''Usage and biology'''</h1> | <h1>'''Usage and biology'''</h1> | ||
| − | It encodes L-lactate permease and D-lactate dehydrogenase, which could produce and transport lactate | + | It encodes L-lactate permease and D-lactate dehydrogenase, which could produce and transport lactate. ldhA could convert pyruvate to D-lactate, lldP could transport lactate out of the cell. In this way, Rhodopseudomonas palustris could effciently transport produced lactate out of the cell, which later will be used by Shewanella. |
<h1>'''Characterization'''</h1> | <h1>'''Characterization'''</h1> | ||
Revision as of 16:56, 17 October 2018
RBS-ldhA-RBS-lldP-TT
L-lactate permease & D-lactate dehydrogenase
Usage and biology
It encodes L-lactate permease and D-lactate dehydrogenase, which could produce and transport lactate. ldhA could convert pyruvate to D-lactate, lldP could transport lactate out of the cell. In this way, Rhodopseudomonas palustris could effciently transport produced lactate out of the cell, which later will be used by Shewanella.
Characterization
This is one section for lactate production and transportation part.
DNA Gel Electrophoretic
To make sure that we get the target gene, we did DNA gel electrophoretic for verification. And here is the result.
Our target genes are 2739bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes.
Electrogenesis
By detecting the production of lactate after expressing protein, we might find out whether RBS-ldhA-RBS-lldP-tt could effectively help Rhodopseudomonas palustris to produce more lactate.
It could be demonstrated that targeted genes could be expressed in the engineered cells. More lactate has been produced by engineered bacteria.