Difference between revisions of "Part:BBa K2558215:Design"
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NEON CRISPRi Safety Catch device with lux pR promotor
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__NOTOC__
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<partinfo>BBa_K2558215 short</partinfo>
 
<partinfo>BBa_K2558215 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2558215 SequenceAndFeatures</partinfo>
  
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===Design Note===
 
===Design Note===
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In order to investigate how lacI dosage affect IPTG induction we used Anderson promotor J23100, J23110 and J23114 to design three constitutive lacI generator of different intensity. The three lacI generator were then ligated with Ptac controlled reporter sfGFP to make three IPTG induction devices (https://parts.igem.org/Part:BBa_K2558203, https://parts.igem.org/Part:BBa_K2558204, https://parts.igem.org/Part:BBa_K2558205). By measuring sfGFP fluorescence we tested how these devices react to IPTG.
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Assisted with the experiences we gained form the experiments above, we built and tuned the NEON system. We designed this experiment to characterize how Neon the positive feedback plasmid (BBa_K2558214), and Safety Catch the CRISPRi plasmid (BBa_K2558215, BBa_K2558216) work together.
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===Source===
 
===Source===

Latest revision as of 16:45, 17 October 2018

CRISPRi Safety Catch device with lux pR promotor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1163
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5547
    Illegal BamHI site found at 3442
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5777
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4314


Design Note

Assisted with the experiences we gained form the experiments above, we built and tuned the NEON system. We designed this experiment to characterize how Neon the positive feedback plasmid (BBa_K2558214), and Safety Catch the CRISPRi plasmid (BBa_K2558215, BBa_K2558216) work together.

Source

References