Difference between revisions of "Part:BBa K2587000"
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<partinfo>BBa_K2587000 short</partinfo>
 
<partinfo>BBa_K2587000 short</partinfo>
  
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LuxI is an acyl homoserine lactone synthase, most known from the bacterium <i>Alivibrio fischeri</i>. LuxI is a basic component of the quorum sensing system of <i>A.fischeri</i>. In bacteria it is used as a communication module among those organisms to regulate expression. In this case the synthase is codon optimized for the common yeast, <i>Saccharomyces cerevisiae</i>. Moreover besides the codon optimization the construct contains also type II S cutting sites (BsaI), useful for example for Golden Gate cloning method. In yeast quorum sensing system is not frequently encountered and therefore a codon optimized form for utilization in an eukaryotic organism represents a beginning to design control systems in this type of organisms. For example, this LuxI is coupled in our case with the design of a synthetic promoter, which is supposed to be activated by binding of the quorum sensing molecule together with the regulator (LuxR) to the promoter to induce expression of a reporter or a lysis gene to control cell population.  
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LuxI is an acyl homoserine lactone synthase, most known from the bacterium <i>Alivibrio fischeri</i>. LuxI is a basic component of the quorum sensing system of <nobr><i>A. fischeri</I></nobr>. In bacteria use it as a communication module among organisms to regulate expression of genes. In this part the synthase is codon optimized for the common yeast, <nobr><i>Saccharomyces cerevisiae</I></nobr>. Besides the codon optimization the construct contains also type II S cutting sites (BsaI), useful for example for Golden Gate cloning method. In yeast quorum sensing system is not frequently encountered and therefore a codon optimized form for utilization in an eukaryotic organism represents a beginning to design control systems in this type of organisms. For example, this LuxI is coupled with the design of a synthetic promoter, which is supposed to be activated by binding of the quorum sensing molecule together with the regulator (LuxR) to the promoter to induce expression of a reporter or a lysis gene to control cell population.  
  
  

Revision as of 16:40, 17 October 2018


luxI_codon optimized S.cerevisiae

LuxI is an acyl homoserine lactone synthase, most known from the bacterium Alivibrio fischeri. LuxI is a basic component of the quorum sensing system of <nobr>A. fischeri</nobr>. In bacteria use it as a communication module among organisms to regulate expression of genes. In this part the synthase is codon optimized for the common yeast, <nobr>Saccharomyces cerevisiae</nobr>. Besides the codon optimization the construct contains also type II S cutting sites (BsaI), useful for example for Golden Gate cloning method. In yeast quorum sensing system is not frequently encountered and therefore a codon optimized form for utilization in an eukaryotic organism represents a beginning to design control systems in this type of organisms. For example, this LuxI is coupled with the design of a synthetic promoter, which is supposed to be activated by binding of the quorum sensing molecule together with the regulator (LuxR) to the promoter to induce expression of a reporter or a lysis gene to control cell population.


Usage and Biology

This part can be used as a component for designing synthetic circuits in S.cerevisiae. For instance in our project we used this gene to induce expression of a reporter after activation of a synthetic, self designed promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 6
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 689
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 30
    Illegal BsaI.rc site found at 673