Difference between revisions of "Part:BBa K2533034"
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To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result.
 
To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result.
 
[[File:T--HUST-China--2018-Notebook-gel3.jpeg|400px|thumb|center|Figure2:Verification of successful transformation of pYYDT-mdh]]
 
[[File:T--HUST-China--2018-Notebook-gel3.jpeg|400px|thumb|center|Figure2:Verification of successful transformation of pYYDT-mdh]]
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Our target genes are 1145bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes.
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Our target gene is 1145bp, and as the marker is DS5000, we could be sure that the bright band in this picture is our target gene.
  
 
<h2>Real-Time Quantitative PCR</h2>
 
<h2>Real-Time Quantitative PCR</h2>

Revision as of 15:49, 17 October 2018


RBS-mdh

It encodes NAD dependent malate dehydrogenase.

Usage and biology

It encodes NAD dependent malate dehydrogenase which transforms malate into pyruvate. With the overexpression of mdh, Shewanella could produce NADH more efficiently, which brings more electricity being produced.

Characterization

This is one section for NADH production part.

Figure1:RBS-mdh

DNA Gel Electrophoretic

To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result.

Figure2:Verification of successful transformation of pYYDT-mdh

Our target gene is 1145bp, and as the marker is DS5000, we could be sure that the bright band in this picture is our target gene.

Real-Time Quantitative PCR

To demonstrate that mdh could be overexpressed by engineered Shewanella, we did Real-Time Quantitative PCR.

Figure3:Relative expression level of mdh in engineered Shewanella Oneidensis MR-1.

As we can see from this figure, mdh could be overexpressed by engineered Shewanella.