Difference between revisions of "Part:BBa K2627000"
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<partinfo>BBa_K2627000 short</partinfo>
 
<partinfo>BBa_K2627000 short</partinfo>
  
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CcaS is a protein in CcaS-CcaR two-component system, which is sensitive to green light and red light. CcaS is located on the cell membrane and when it senses the existence of green light or red light, it will experience autophosphorylation and thus phosphorylate the downstream CcaR, and CcaR will bind to specific promoter to activate gene transcription. Typically, gene will be activated under green light and repressed under red light, but this advanced CcaS with PAS domain knocked out has reverse effect, gene will be repressed under green light and activated under red light. In our project, this advanced two-component system is used as a switch to sense different light and turn on the gene to produce PHB.
 
  
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CcaS is a protein in CcaS-CcaR two-component system, which is sensitive to green light and red light. CcaS is located on the cell membrane and when it senses the existence of green light or red light, it will experience autophosphorylation and thus phosphorylate the downstream CcaR, and CcaR will bind to specific promoter to activate gene transcription. Typically, gene will be activated under green light and repressed under red light, but this advanced CcaS with PAS domain knocked out has reverse effect, gene will be repressed under green light and activated under red light. In our project, this advanced two-component system is used as a switch to sense different light and turn on the gene to produce PHB.
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[[File:T--SDU-China--3410.png|800px|center]]
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 15:44, 17 October 2018


Advanced CcaS with PAS domain knocked out


CcaS is a protein in CcaS-CcaR two-component system, which is sensitive to green light and red light. CcaS is located on the cell membrane and when it senses the existence of green light or red light, it will experience autophosphorylation and thus phosphorylate the downstream CcaR, and CcaR will bind to specific promoter to activate gene transcription. Typically, gene will be activated under green light and repressed under red light, but this advanced CcaS with PAS domain knocked out has reverse effect, gene will be repressed under green light and activated under red light. In our project, this advanced two-component system is used as a switch to sense different light and turn on the gene to produce PHB.

T--SDU-China--3410.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 606
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Results

T--SDU-China--4.png

Figure 1: Characterization of part BBa_K2627000 and BBa_K592001. Part BBa_K2627000 shows a different effect that the index under red light is higher than the index under green light. The Fluorescence intensity is about 1207.4 under green light while it is about 3146.3 under red light. For this reason, we can treat the index under red light as expression index and treat the index under green light as leak index.