Difference between revisions of "Part:BBa K2762015"

(→‎Characterization)
(→‎Characterization)
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===Characterization===
 
===Characterization===
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This composite part includes a <i>P<sub>gadA</sub></i> (K1231001), a <i>RBS</i> (B0034) and a <i>GFP</i> (K2762014).Promoter gadA is a pH sensitive promoter from <i>E. coli</i> and <i>gadA</i> is one of the enzyme which is from glutamate decarboxylase (GAD) system. The expression of all of the <i>gad</i> genes in <i>E. coli</i>, including <i>gadA</i> is rely on the alternate sigma factor which encoded by <i>rpoS</i>, and repressed by the cAMP receptor protein and H-NS.
+
This composite part includes a <i>P<sub>gadA</sub></i> (K1231001), a <i>RBS</i> (B0030) and a <i>GFP</i> (E0040).Promoter gadA is a pH sensitive promoter from <i>E. coli</i> and <i>gadA</i> is one of the enzyme which is from glutamate decarboxylase (GAD) system. The expression of all of the <i>gad</i> genes in <i>E. coli</i>, including <i>gadA</i> is rely on the alternate sigma factor which encoded by <i>rpoS</i>, and repressed by the cAMP receptor protein and H-NS.
  
  

Revision as of 15:40, 17 October 2018


PgadA-B0034-GFP

Characterization

This composite part includes a PgadA (K1231001), a RBS (B0030) and a GFP (E0040).Promoter gadA is a pH sensitive promoter from E. coli and gadA is one of the enzyme which is from glutamate decarboxylase (GAD) system. The expression of all of the gad genes in E. coli, including gadA is rely on the alternate sigma factor which encoded by rpoS, and repressed by the cAMP receptor protein and H-NS.


T--NCKU Tainan--part BBa K2762015 new .png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 937