Difference between revisions of "Part:BBa K2534007:Design"
Line 2: | Line 2: | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K2534007 short</partinfo> | <partinfo>BBa_K2534007 short</partinfo> | ||
+ | |||
+ | [http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] managed to improve AmpR at [https://parts.igem.org/Part:BBa_K2308011 # BBa_K2308011] by fusing AmpR to AmpR promoter which was found to be RhoN and constitutively essential for plasmid selection in lentiviral delivery transfer plasmids. | ||
+ | |||
+ | [http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] have characterized this part considering it's apoptotic effect of RKO cell lines. | ||
+ | |||
+ | https://static.igem.org/mediawiki/parts/2/29/ChAMPRAFCM.png | ||
+ | |||
+ | |||
+ | For further information about demonstration of its apoptotic effect, please visit our results page | ||
+ | http://2018.igem.org/Team:AFCM-Egypt/Demonstrate | ||
+ | For further information about design of our parts, please visit our Parts & Design Page | ||
+ | http://2018.igem.org/Team:AFCM-Egypt/Parts | ||
<partinfo>BBa_K2534007 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2534007 SequenceAndFeatures</partinfo> |
Latest revision as of 15:36, 17 October 2018
AMPR promoter + AMPR
[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] managed to improve AmpR at # BBa_K2308011 by fusing AmpR to AmpR promoter which was found to be RhoN and constitutively essential for plasmid selection in lentiviral delivery transfer plasmids.
[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] have characterized this part considering it's apoptotic effect of RKO cell lines.
For further information about demonstration of its apoptotic effect, please visit our results page
http://2018.igem.org/Team:AFCM-Egypt/Demonstrate
For further information about design of our parts, please visit our Parts & Design Page
http://2018.igem.org/Team:AFCM-Egypt/Parts
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 254
Design Notes
IDT syntheised
Source
IDT synthesised