Difference between revisions of "Part:BBa K2534007:Design"

 
 
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<partinfo>BBa_K2534007 short</partinfo>
 
<partinfo>BBa_K2534007 short</partinfo>
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[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] managed to improve AmpR at [https://parts.igem.org/Part:BBa_K2308011 # BBa_K2308011] by fusing AmpR to AmpR promoter which was found to be RhoN and constitutively essential for plasmid selection in lentiviral delivery transfer plasmids.
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[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] have characterized this part considering it's apoptotic effect of RKO cell lines.
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https://static.igem.org/mediawiki/parts/2/29/ChAMPRAFCM.png
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For further information about demonstration of its apoptotic effect, please visit our results page
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http://2018.igem.org/Team:AFCM-Egypt/Demonstrate
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For further information about design of our parts, please visit our Parts & Design Page
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http://2018.igem.org/Team:AFCM-Egypt/Parts
  
 
<partinfo>BBa_K2534007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2534007 SequenceAndFeatures</partinfo>

Latest revision as of 15:36, 17 October 2018


AMPR promoter + AMPR

[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] managed to improve AmpR at # BBa_K2308011 by fusing AmpR to AmpR promoter which was found to be RhoN and constitutively essential for plasmid selection in lentiviral delivery transfer plasmids.

[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] have characterized this part considering it's apoptotic effect of RKO cell lines.

ChAMPRAFCM.png


For further information about demonstration of its apoptotic effect, please visit our results page http://2018.igem.org/Team:AFCM-Egypt/Demonstrate For further information about design of our parts, please visit our Parts & Design Page http://2018.igem.org/Team:AFCM-Egypt/Parts


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 254


Design Notes

IDT syntheised


Source

IDT synthesised

References