Difference between revisions of "Part:BBa K2533049"

 
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L-lactate permease & D-lactate dehydrogenase
 
L-lactate permease & D-lactate dehydrogenase
  
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<h1>'''Usage and biology'''</h1>
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===Usage and Biology===
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dld refers to FAD-dependent D-lactate dehydrogenase which could catalyze D-lactate’s transformation into pyruvate. With the overexpression of dld, Shewanella could utilize D-lactate more efficiently, which brings more electricity being produced.
  
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<h1>'''Characterization'''</h1>
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<span class='h3bb'>Sequence and Features</span>
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This is one section for lactate utilization part.
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<partinfo>BBa_K2533049 SequenceAndFeatures</partinfo>
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[[File:T--HUST-China--2018-tonglu-lldP-ldhA.png ‎|400px|thumb|center|Figure1:RBS-lldP-RBS-ldhA]]
  
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<h2>DNA Gel Electrophoretic</h2>
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To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result.
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[[File:T--HUST-China--2018-jiaotu-lldP-ldhA.png|400px|thumb|center|Figure2:Verification of successful transformation of pSB1C3-RBS-lldP-RBS-ldhA]]
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Our target genes are 2690bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes.
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<h2>Electrogenesis</h2>
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By comparing the ability of producing electricity, we might find out whether dld could effectively help Shewanella to produce more electricity.
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[[File:T--HUST-China--2018-expression of lactate.png ‎|400px|thumb|center|Figure3:shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits achieve the goal to help strains produce lactate.]]
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It could be demonstrated that targeted genes could be expressed in the engineered cells. More NADH has been produced by engineered bacteria, which helps to produce more electricty.
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2533049 parameters</partinfo>
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<partinfo>BBa_K2533030 parameters</partinfo>
 
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Revision as of 15:29, 17 October 2018


RBS-lldP-RBS-ldhA

L-lactate permease & D-lactate dehydrogenase

Usage and biology

dld refers to FAD-dependent D-lactate dehydrogenase which could catalyze D-lactate’s transformation into pyruvate. With the overexpression of dld, Shewanella could utilize D-lactate more efficiently, which brings more electricity being produced.

Characterization

This is one section for lactate utilization part.

Figure1:RBS-lldP-RBS-ldhA

DNA Gel Electrophoretic

To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result.

Figure2:Verification of successful transformation of pSB1C3-RBS-lldP-RBS-ldhA

Our target genes are 2690bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes.

Electrogenesis

By comparing the ability of producing electricity, we might find out whether dld could effectively help Shewanella to produce more electricity.

Figure3:shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits achieve the goal to help strains produce lactate.

It could be demonstrated that targeted genes could be expressed in the engineered cells. More NADH has been produced by engineered bacteria, which helps to produce more electricty.