Difference between revisions of "Part:BBa K2533033"
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To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result. | To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result. | ||
[[File:T--HUST-China--2018-Notebook-gel3.jpeg|400px|thumb|center|Figure2:Verification of successful transformation of pYYDT-gapA]] | [[File:T--HUST-China--2018-Notebook-gel3.jpeg|400px|thumb|center|Figure2:Verification of successful transformation of pYYDT-gapA]] | ||
| − | Our target | + | Our target gene is 1126bp, and as the marker is DS5000, we could be sure that the bright band in this picture is our target gene. |
<h2>Real-Time Quantitative PCR</h2> | <h2>Real-Time Quantitative PCR</h2> | ||
Revision as of 15:26, 17 October 2018
RBS-gapA
It encodes glyceraldehyde-3-phosphate dehydrogenase.
Usage and biology
It encodes glyceraldehyde-3-phosphate dehydrogenase which could transform 3- phosphoglyceraldehyde into 1,3- diphosphoglycerate. With the overexpression of gapA, Shewanella could produce NADH more efficiently, which brings more electricity being produced.
Characterization
This is one section for NADH production part.
DNA Gel Electrophoretic
To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result.
Our target gene is 1126bp, and as the marker is DS5000, we could be sure that the bright band in this picture is our target gene.
Real-Time Quantitative PCR
To demonstrate that gapA could be overexpressed by engineered Shewanella, we did Real-Time Quantitative PCR.
There was no signal in bacteria which contained pYYDT so we chose pYYDT-gapA as standard quantity.