Difference between revisions of "Part:BBa K2632006"
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| − | Pyroptosis is a form of lytic programmed cell death with inflammation. Recent studies reported that N-terminal of | + | Pyroptosis is a form of lytic programmed cell death with inflammation. Recent studies reported that N-terminal of Gasdermin D <a href="https://parts.igem.org/Part:BBa_K2632003">(BBa K2632003)</a> acts as a effector of pyroptosis. Full length Gasdermin D is cleaved by Caspase 1 then release the PFD (pore-forming domain) which can oligomerize on the cell membrane. Formation of pore causes cell swelling, rupture of the membrane and massive leakage of cytosolic contents. Y373D mutation of full length Gasdermin D reduces the cell toxicity. |
<h3>Reference</h3> | <h3>Reference</h3> | ||
Latest revision as of 15:18, 17 October 2018
Y373D mutant of full length Gasdermin D.
Pyroptosis is a form of lytic programmed cell death with inflammation. Recent studies reported that N-terminal of Gasdermin D (BBa K2632003) acts as a effector of pyroptosis. Full length Gasdermin D is cleaved by Caspase 1 then release the PFD (pore-forming domain) which can oligomerize on the cell membrane. Formation of pore causes cell swelling, rupture of the membrane and massive leakage of cytosolic contents. Y373D mutation of full length Gasdermin D reduces the cell toxicity.
Reference
Ding J, Wang K, Liu W, et al. Pore-forming activity and structural autoinhibition of the gasdermin family[J]. Nature, 2016, 535(7610):111.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1204
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 883