Difference between revisions of "Part:BBa K2622038"

 
 
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<partinfo>BBa_K2622038 short</partinfo>
 
<partinfo>BBa_K2622038 short</partinfo>
  
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Mstx-scFv long description
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Mstx-scFv has a Mstx protein (BBa_K2622006) fused to a N-terminus of ScFv-antiVGY (BBa_K2622004). As single chains tend to aggregate when expressed in E. coli, Mstx anchor could prevent by stabilizing single chains and therefore increasing overall yield of ScFv-antiVGY expression.
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This composite part has a RFC10 prefix and suffix, though it has an incompatible XbaI site between T7 promoter and RBS that must be avoided.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:46, 17 October 2018


Mstx-scFv

Mstx-scFv has a Mstx protein (BBa_K2622006) fused to a N-terminus of ScFv-antiVGY (BBa_K2622004). As single chains tend to aggregate when expressed in E. coli, Mstx anchor could prevent by stabilizing single chains and therefore increasing overall yield of ScFv-antiVGY expression.

This composite part has a RFC10 prefix and suffix, though it has an incompatible XbaI site between T7 promoter and RBS that must be avoided.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 39
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 39
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 39
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 458
    Illegal BsaI.rc site found at 20