Difference between revisions of "Part:BBa K2533002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | LdhDnARSdR is ldhD (lactate dehydrogenase gene) with D176A/I177R/F178S/N180R. LldP protein gene is used to transport the lactate out of the cell. We successfully construct genes ldhDnARSdR-lldp using molecular biology experiment methods. | |
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | [1]Li, C., Tao, F., Ni, J., Wang, Y., Yao, F., & Xu, P. (2015). Enhancing the light-driven production of d-lactate by engineering cyanobacterium using a combinational strategy. Scientific Reports, 5, 9777. http://doi.org/10.1038/srep09777 | ||
Latest revision as of 14:31, 17 October 2018
RBS-ldhDnARSdR-RBS-lldP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 780
Illegal BglII site found at 1325 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1461
Illegal AgeI site found at 1924
Illegal AgeI site found at 2362
Illegal AgeI site found at 2521
Illegal AgeI site found at 2533 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 764
Illegal BsaI.rc site found at 1035
Design Notes
LdhDnARSdR is ldhD (lactate dehydrogenase gene) with D176A/I177R/F178S/N180R. LldP protein gene is used to transport the lactate out of the cell. We successfully construct genes ldhDnARSdR-lldp using molecular biology experiment methods.
Source
From Lactobacillus bulgaricus.
References
[1]Li, C., Tao, F., Ni, J., Wang, Y., Yao, F., & Xu, P. (2015). Enhancing the light-driven production of d-lactate by engineering cyanobacterium using a combinational strategy. Scientific Reports, 5, 9777. http://doi.org/10.1038/srep09777