Difference between revisions of "Part:BBa K2558007"

Latest revision as of 14:28, 17 October 2018

gRNA targeting lux pR and RiboJ

gRNA targeting lux pR and RiboJ is a sequence used in our NEON CRISPRi Safety Catch device (K2558214, K2558215), whose function is to guide dCas9 to the lux pR promoter and therefore inhibit the expression of downstream genes (luxI and GFP). Even though the lacI on the Safety Catch is also controlled by luxpR, this design of gRNA whose targeting sequence also includes part of the sequence of RiboJ allows us to make the lux pR in Safety Catch, which is not followed by RiboJ, unaffected by CRISPRi. Therefore lux pR on Safety Catch can still be activated by luxR-AHL complex.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 17
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2558007 short</partinfo>
-gRNA targeting luxpR and RiboJ
+gRNA targeting lux pR and RiboJ is a sequence used in our NEON CRISPRi Safety Catch device (K2558214, K2558215), whose function is to guide dCas9 to the lux pR promoter and therefore inhibit the expression of downstream genes (luxI and GFP). Even though the lacI on the Safety Catch is also controlled by luxpR, this design of gRNA whose targeting sequence also includes part of the sequence of RiboJ allows us to make the lux pR in Safety Catch, which is not followed by RiboJ, unaffected by CRISPRi. Therefore lux pR on Safety Catch can still be activated by luxR-AHL complex.
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-<span class='h3bb'>Sequence and Features</span>
+===Sequence and Features===
 <partinfo>BBa_K2558007 SequenceAndFeatures</partinfo>