Difference between revisions of "Part:BBa K2622033"
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<partinfo>BBa_K2622033 short</partinfo> | <partinfo>BBa_K2622033 short</partinfo> | ||
− | His- | + | His-ScFv-antiVGY has a 6xHis-tag attached to ScFv-antiVGY's (BBa_K2622004) N-terminus. This part could be used to purify ScFv-antiVGY prior to erythrocyte membrane lysis assay or to perform ELISA with ScFv-antiVGY. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 14:16, 17 October 2018
His-igA
His-ScFv-antiVGY has a 6xHis-tag attached to ScFv-antiVGY's (BBa_K2622004) N-terminus. This part could be used to purify ScFv-antiVGY prior to erythrocyte membrane lysis assay or to perform ELISA with ScFv-antiVGY.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 198
Illegal XbaI site found at 39
Illegal PstI site found at 181 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 198
Illegal NheI site found at 108
Illegal PstI site found at 181 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 198
Illegal BglII site found at 177
Illegal BamHI site found at 164
Illegal XhoI site found at 173 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 198
Illegal XbaI site found at 39
Illegal PstI site found at 181 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 198
Illegal XbaI site found at 39
Illegal PstI site found at 181
Illegal NgoMIV site found at 1041
Illegal AgeI site found at 816 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 20