Difference between revisions of "Part:BBa K2789001:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | This year we have improved the part BBa_K608102 designed by the 11-Freiburg iGEM team. This is a light control component Ccas. We want to use this component, but there are illegal EcoRI and SpeI sites in the sequence that do not comply with the RFC10 standard. Since this component needs to be used with another part Ccar, there will be problems during the assembly process. This team also encountered this problem, and this part has not been characterized. | |
− | + | ||
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All the parts come from igem registry resources. | All the parts come from igem registry resources. | ||
− | Ccas: | + | |
− | Ccar: | + | Ccas: BBa_K608102 |
+ | |||
+ | Ccar: BBa_K592002 | ||
+ | |||
Lac operator: BBa_C0010, BBa_C0012 | Lac operator: BBa_C0010, BBa_C0012 | ||
+ | |||
double terminator: BBa_B0015 | double terminator: BBa_B0015 | ||
+ | rbs: BBa_B0034 | ||
===References=== | ===References=== | ||
+ | |||
+ | We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102:Design) | ||
+ | |||
+ | And this part BBa_K2789001 is consists of lac operator , Ccas and Ccar (The downstream signal transducers to control gene expression) |
Latest revision as of 14:09, 17 October 2018
lac operator+rbs+Ccas+terminator+Ccar+lac operator+rbs+terminator
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1393
Illegal BamHI site found at 2973 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 798
Illegal AgeI site found at 1803
Illegal AgeI site found at 1998 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This year we have improved the part BBa_K608102 designed by the 11-Freiburg iGEM team. This is a light control component Ccas. We want to use this component, but there are illegal EcoRI and SpeI sites in the sequence that do not comply with the RFC10 standard. Since this component needs to be used with another part Ccar, there will be problems during the assembly process. This team also encountered this problem, and this part has not been characterized.
Source
All the parts come from igem registry resources.
Ccas: BBa_K608102
Ccar: BBa_K592002
Lac operator: BBa_C0010, BBa_C0012
double terminator: BBa_B0015
rbs: BBa_B0034
References
We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102:Design)
And this part BBa_K2789001 is consists of lac operator , Ccas and Ccar (The downstream signal transducers to control gene expression)