Difference between revisions of "Part:BBa K2595009"
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<partinfo>BBa_K2595009 short</partinfo> | <partinfo>BBa_K2595009 short</partinfo> | ||
| − | - | + | SgrS (wt) is a small regulatory sRNA that controls the expression of the PtsG gene in Escherichia coli K-12. The PtsG gene codes for the major glucose transporter, which is necessary for the transport of glucose into the cytoplasm. SgrS action is dependent of Hfq, a chaperone that interacts with the sRNA at its hairpin loop and poly U tail. This stabilizes the interaction of the 3’ end of SgrS with the Shine Dalgarmo sequence of ptsG mRNA by partial complementary base-pairing (Bobrovskyy and Vanderpool, 2014). Upon successful formation of the sRNA-mRNA duplex complex, the formation of the ribosomal machinery is inhibited, therefore blocking translation. |
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This synthetic version of SgrS follow the same principles as its wild type partner to down-regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, SgrS can down-regulate the expression of the gene that contains the complementary regions for its targeting sequence. Ligation with the desired targeting sequence does not create any scar in the sRNA sequence, therefore allowing its use for a broad range of gene targets as well as with any biobricks that may benefit from its functions. | ||
| − | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2595009 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2595009 SequenceAndFeatures</partinfo> | ||
| − | + | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K2595009 parameters</partinfo> | <partinfo>BBa_K2595009 parameters</partinfo> | ||
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Revision as of 13:34, 17 October 2018
SgrS
SgrS (wt) is a small regulatory sRNA that controls the expression of the PtsG gene in Escherichia coli K-12. The PtsG gene codes for the major glucose transporter, which is necessary for the transport of glucose into the cytoplasm. SgrS action is dependent of Hfq, a chaperone that interacts with the sRNA at its hairpin loop and poly U tail. This stabilizes the interaction of the 3’ end of SgrS with the Shine Dalgarmo sequence of ptsG mRNA by partial complementary base-pairing (Bobrovskyy and Vanderpool, 2014). Upon successful formation of the sRNA-mRNA duplex complex, the formation of the ribosomal machinery is inhibited, therefore blocking translation.
Usage and Biology
This synthetic version of SgrS follow the same principles as its wild type partner to down-regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, SgrS can down-regulate the expression of the gene that contains the complementary regions for its targeting sequence. Ligation with the desired targeting sequence does not create any scar in the sRNA sequence, therefore allowing its use for a broad range of gene targets as well as with any biobricks that may benefit from its functions.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]