Difference between revisions of "Part:BBa K2622026"

Latest revision as of 13:30, 17 October 2018

Lpp-OmpA-His

Lpp-OmpA-His has a 6x His-tag attached to Lpp-OmpA's (BBa_K1991004) C-terminus via glycine/serine linker, so that it could be displayed and detected in the outer surface of the membrane. Lpp protein does not have a signal sequence as this part was designed to be used in vitro systems (liposomes, nanodiscs). This part should be expressed in IVTT's or bacteria lysates based on a T7 RNR polymerase as it has T7 promoter and terminator.

This composite part has a RFC10 prefix and suffix, though it has an incompatible XbaI site between T7 promoter and RBS that must be avoided.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 39
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 456
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 39
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 39
Illegal NgoMIV site found at 411
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 20

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2622026 short</partinfo>
-Lpp-OmpA-His long description
+Lpp-OmpA-His has a 6x His-tag attached to Lpp-OmpA's (BBa_K1991004) C-terminus via glycine/serine linker, so that it could be displayed and detected in the outer surface of the membrane. Lpp protein does not have a signal sequence as this part was designed to be used in vitro systems (liposomes, nanodiscs). This part should be expressed in IVTT's or bacteria lysates based on a T7 RNR polymerase as it has T7 promoter and terminator.
+This composite part has a RFC10 prefix and suffix, though it has an incompatible XbaI site between T7 promoter and RBS that must be avoided.
 <!-- Add more about the biology of this part here