Difference between revisions of "Part:BBa K2800027"

 
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===Usage and Biology===
 
===Usage and Biology===
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<h1><b>Biology:
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<p>&nbsp;&nbsp;&nbsp;&nbsp;This part is inducible promoter Ptet which is induced by tetracycline. This is the basic principle of Tet induced expression system, it is to regulate the conformation of proteins by inducer such as Tet, thus controlling the expression of target proteins. When there is no tetracycline, TetR will bind to TetO to block downstream expression of resistant genes. When tetracycline is added, it will bind to TetR and change its conformation, then TetR is separated from TetO, thereby relieving the inhibition of resistance genes.
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<img src="https://static.igem.org/mediawiki/2018/4/4b/T--HUBU-Wuhan--part1.png" alt="" style="text-align: center;width:60%;padding:0 0;">
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<p style="text-align: center;padding:0 0;">Fig.1 The principle of Tet and TetR/TetO system. 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2800027 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2800027 SequenceAndFeatures</partinfo>

Revision as of 12:56, 17 October 2018


Ptet promoter

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Usage and Biology

Biology:

    This part is inducible promoter Ptet which is induced by tetracycline. This is the basic principle of Tet induced expression system, it is to regulate the conformation of proteins by inducer such as Tet, thus controlling the expression of target proteins. When there is no tetracycline, TetR will bind to TetO to block downstream expression of resistant genes. When tetracycline is added, it will bind to TetR and change its conformation, then TetR is separated from TetO, thereby relieving the inhibition of resistance genes.

Fig.1 The principle of Tet and TetR/TetO system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]