Difference between revisions of "Part:BBa K2686005:Experience"
 
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The part was designed and constructed in the original pET14 vector from <bbpart>K2686001</bbpart>. The sequence was confirmed using Sanger sequencing.
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Since this part is an intermediary only built for cloning purposes, the part was never expressed by itself in cell-free, but was ligated with inserts (OT1 coding sequences in our case) using Golden Gate assemblies with BsaI.
  
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing.
 
 
===Applications of BBa_K2686005===
 
===Applications of BBa_K2686005===
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The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing. The part was then amplified out of the pET vector and inserted into the pSB1C3 plasmid backbone, and submitted. There was not enough time to perform Sanger sequencing, but colony PCR gave us the correct insert size.
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===User Reviews===
 
===User Reviews===
 
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<partinfo>BBa_K2686005 AddReview number</partinfo>
 
<partinfo>BBa_K2686005 AddReview number</partinfo>
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<I>Username</I>
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<I>m_houmani_EPFL2018</I>
 
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Enter the review inofrmation here.
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|};
 
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Latest revision as of 12:50, 17 October 2018

The part was designed and constructed in the original pET14 vector from K2686001. The sequence was confirmed using Sanger sequencing. Since this part is an intermediary only built for cloning purposes, the part was never expressed by itself in cell-free, but was ligated with inserts (OT1 coding sequences in our case) using Golden Gate assemblies with BsaI.

Applications of BBa_K2686005

User Reviews

UNIQ6d5e27a8385298ab-partinfo-00000001-QINU UNIQ6d5e27a8385298ab-partinfo-00000002-QINU