Difference between revisions of "Part:BBa K2719000:Experience"
(→Applications of BBa_K2719000) |
|||
| Line 9: | Line 9: | ||
[[file:T--TecCEM--GSTColonies.png|500px]] | [[file:T--TecCEM--GSTColonies.png|500px]] | ||
| − | <p>Figure 1. Transformed GST Colonies</p> | + | <p><i>Figure 1.</i> Transformed GST Colonies</p> |
To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the entire plasmid and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of GST. (Figure 4 and 3) | To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the entire plasmid and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of GST. (Figure 4 and 3) | ||
Revision as of 12:49, 17 October 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2719000
To confirm the presence of GST, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in Escherichia coli DH5a. (Figure 1)
Figure 1. Transformed GST Colonies
To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the entire plasmid and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of GST. (Figure 4 and 3)
User Reviews
UNIQd2229360324738c7-partinfo-00000000-QINU UNIQd2229360324738c7-partinfo-00000001-QINU