Difference between revisions of "Part:BBa K2686006:Experience"
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| + | The part was designed and constructed in the original pET14 vector from <bbpart>K2686001</bbpart>. The sequence was confirmed using Sanger sequencing. The part was expressed in the pET14 vector using a BL21 DE3 TX-TL (Sun et al., 2013) cell free expression system. After expression and purification, the part was validated in multiple ways. First, multiple SDS-PAGE experiments indicate that our expressed protein is at the correct hypothetical size, for the 60-mer and that sfGFP binds to the encapsulin. | ||
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===Applications of BBa_K2686006=== | ===Applications of BBa_K2686006=== | ||
Latest revision as of 12:49, 17 October 2018
The part was designed and constructed in the original pET14 vector from K2686001. The sequence was confirmed using Sanger sequencing. The part was expressed in the pET14 vector using a BL21 DE3 TX-TL (Sun et al., 2013) cell free expression system. After expression and purification, the part was validated in multiple ways. First, multiple SDS-PAGE experiments indicate that our expressed protein is at the correct hypothetical size, for the 60-mer and that sfGFP binds to the encapsulin.
Applications of BBa_K2686006
User Reviews
UNIQebc948a70d07aaa0-partinfo-00000001-QINU UNIQebc948a70d07aaa0-partinfo-00000002-QINU