Difference between revisions of "Part:BBa K2719008:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K2719008 short</partinfo> | <partinfo>BBa_K2719008 short</partinfo> | ||
| + | <p>This is originated from <i>Hommo sapiens</i>, the original sequence is from this species but was optimized for <i>E.coli</i> BL21 (DE3) and to be cloned by RFC10 on pSB1C3. It mainly consists on a-helix. An histidine tag (6xHis) was added to the protein to facilitate its purification.</p> | ||
| + | "https://static.igem.org/mediawiki/2018/d/d3/T--TecCEM--Lep3DStructure.png" | ||
| + | <p><i>Figure 7.</i> Leptin 3D prediction modelled with Swiss Model</p> | ||
<partinfo>BBa_K2719008 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2719008 SequenceAndFeatures</partinfo> | ||
Revision as of 12:44, 17 October 2018
Leptin
This is originated from Hommo sapiens, the original sequence is from this species but was optimized for E.coli BL21 (DE3) and to be cloned by RFC10 on pSB1C3. It mainly consists on a-helix. An histidine tag (6xHis) was added to the protein to facilitate its purification.
"
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Figure 7. Leptin 3D prediction modelled with Swiss Model
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Optimized for E.coli.
Source
NCBI Genomic Library