Difference between revisions of "Part:BBa K2719006:Design"

(→‎Design Notes)
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As in humans collagen requires the hydroxylation of proline for the proper formation of the tertiary structure and its correct function.  Since it is difficult to induce postraductional modifications in a prokaryotic system, this part was designed for the incorporation of hydroxyproline as a non-canonical amino acid.  Thus eliminating the need of post traductional modifications.  In order to allow the incorporation of non-canonical amino acids, this sequence was optimized for E.coli Rosetta (DE3).   
 
As in humans collagen requires the hydroxylation of proline for the proper formation of the tertiary structure and its correct function.  Since it is difficult to induce postraductional modifications in a prokaryotic system, this part was designed for the incorporation of hydroxyproline as a non-canonical amino acid.  Thus eliminating the need of post traductional modifications.  In order to allow the incorporation of non-canonical amino acids, this sequence was optimized for E.coli Rosetta (DE3).   
 +
  
 
"https://static.igem.org/mediawiki/2018/0/0e/T--TecCEM--ColV2DStructure.png"
 
"https://static.igem.org/mediawiki/2018/0/0e/T--TecCEM--ColV2DStructure.png"

Revision as of 12:39, 17 October 2018


Collagen V - Like


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 92


Design Notes

As in humans collagen requires the hydroxylation of proline for the proper formation of the tertiary structure and its correct function. Since it is difficult to induce postraductional modifications in a prokaryotic system, this part was designed for the incorporation of hydroxyproline as a non-canonical amino acid. Thus eliminating the need of post traductional modifications. In order to allow the incorporation of non-canonical amino acids, this sequence was optimized for E.coli Rosetta (DE3).


"T--TecCEM--ColV2DStructure.png"

Figure 6. 2D structure of Collagen V-like

Source

Genomic sequence

References